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paper pcw57 1 addgene david root lab  (Addgene inc)


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    Addgene inc paper pcw57 1 addgene david root lab
    Paper Pcw57 1 Addgene David Root Lab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+pcw57+1+addgene+david+root+lab/pCW57%2E1+(Plasmid+%2341393)/pm40934084-277-107-111
    Average 96 stars, based on 406 article reviews
    paper pcw57 1 addgene david root lab - by Bioz Stars, 2026-11
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    Article Title: Disrupting the interaction between androgen receptor and its coregulator WDR77 delays the growth of treatment-resistant prostate cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 2000 Thermo-Fisher Scientific Cat# 11668019 Fugene HD Promega Cat# E2311 Glutathione Agarose Resin ThermoScientific Cat# 16101 Cytiva CM5 sensor Chip Cytiva Cat# BR100530 Octet® NTA Biosensors Sartorius Cat# 18-5101 Growth factor reduced phenol-free Matrigel Becton-Dickinson Cat# 356231 Matrigel matrix basement membrane Becton-Dickinson Cat# 356234 Critical commercial assays 1-Step Human High-Yield Mini IVT Kit Thermo-Fisher Scientific Cat# 88891 LookOut Mycoplasma PCR Detection Kit Sigma-Aldrich Cat# MP0035 CellTiter Aqueous One Solution Cell Proliferation Assays Promega Cat# G3580 Anti-DYDDDDK magnetic beads Pierce Cat# A36797 Anti-HA agarose beads Pierce Cat# 26181 NEBNext UltraII DNA library prep kit Illumina Cat# E7103S NEBNext UltraII library dual index primers Illumina Cat# E7600S Deposited data Raw and analyzed CUT&RUN seq data This paper GEO(GSE252993) RNAseq raw and analyzed files This paper GEO(GSE287925) Experimental models: Cell lines LNCaP ATCC Cat# CRL-1740 PC3 ATCC Cat# CRL-1435 DU145 ATCC Cat# HTB-81 C4-2 Uro-Cor – R1-D567 Scott Dehm laboratory, University of Minnesota – NIH3T3 Cell services core facility at Cleveland Clinic Lerner Research Institute – RWPE1 ATCC Cat# CRL-3607 RWPE2 ATCC Cat# CRL-3608 HEK293 Cell services core facility at Cleveland Clinic Lerner Research Institute – Experimental models: Organisms/strains homozygous nude (Foxn1nu/Foxn1nu) mice Jackson laboratory Strain 007850 Fox Chase SCID mouse Charles Rivers Laboratory Strain 236 Oligonucleotides Overview of primers used, included in Table S2 This paper N/A siRNAs targeting WDR77 (siGenome SmartPool) Horizon Discovery Cat#M-006895-01-0005 siRNAs targeting AR (siGenome SmartPool) Horizon Discovery Cat#M-003400-02-0005 Non-targeting control siRNAs (siGenome SmartPool) Horizon Discovery Cat#D-001810-10-20 Recombinant DNA p3xFLAG-CMV10 plasmid Sigma-Aldrich Cat# E7658 pT7CFE1-NHA-Chis ThermoFisher Scientific Cat# 88861 pT7CFE1-NFtag ThermoFisher Scientific Cat# 88865 pT7CFE1-NHis GST-CHA ThermoFisher Scientific Cat# 88871 (Continued on next page) Cell Reports 44, 116235, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER SMARTvector 2 inducible human WDR77targeting mCMV-turboGFP shRNA (sequences listed in Table S2) Horizon Discovery V3IHSMCG_4710023, V3IHSMCG_4967357, V3IHSMCG_8182514 SMARTvector inducible non-targeting control shRNA Horizon Discovery Cat# VSC6570 pT7CFE1-NHA-CHis-p53 This paper – pT7CFE1-Nflag-WDR77 This paper – pT7CFE1-Nflag-WDR77 1-107aa This paper – pT7CFE1-Nflag-WDR77 108-207aa This paper – pT7CFE1-Nflag-WDR77 208-342 This paper – pT7CFE1-Nis-GST-CHA-AR This paper – pT7CFE1-Nis-GST-CHA-AR-NTD This paper – pT7CFE1-Nis-GST-CHA-AR-DBD This paper – pT7CFE1-Nis-GST-CHA-AR-LBD This paper – pT7CFE1-Nis-GST-CHA-PRMT5 This paper – p3xFLAG-CMV10-WDR77 This paper – p3xFLAG-CMV10-WDR77 1-107aa This paper – p3xFLAG-CMV10-WDR77 108-207aa This paper – p3xFLAG-CMV10-WDR77 207-342aa This paper – p3xFLAG-CMV10-WDR77 SM This paper – p3xFLAG-CMV10-WDR77 DM This paper – p3xFLAG-CMV10-WDR77 peptide 3 This paper – pCW57.1 – Addgene David Root lab Cat# 41393 pCW57.1-WDR77 peptide 3 This paper – 3XHA-TurboID-NLS-pcDNA3 Kurt Runge lab, Cleveland Clinic – 3XHA-TurboID-NLS-pcDNA3-AR This paper – FUGW – Addgene Lois et al. 49 Cat# 14883 psPAX2 Abhishek Chakraborty lab, Cleveland Clinic – pMD2.G Abhishek Chakraborty lab, Cleveland Clinic – FUGW-WDR77-peptide 3 This paper – FUGW-WDR77-peptide 3 DM This paper – pCMV-AR Steven Balk laboratory, Beth Isreal Deaconess Medical Center – Software and algorithms Fiji (ImageJ) version 1.54 – https://imagej.net/software/fiji/ ChimeraX version 1.4 – https://www.rbvi.ucsf.edu/chimerax/ Image Studio (LI-COR) version 3.1 LI-COR https://www.licorbio.com/image-studio cBIO portal Cerami et al. 21 https://www.cbioportal.org/ Sigmaplot v16 – https://sigmaplot.software.informer.com/ 11.0/ Rstudio v2023.01 – https://www.r-project.org/ Prostate Cancer Transcriptome Atlas (PCTA) You et al. 27 http://www.thepcta.org/ Genomics Analysis pipeline – https://github.com/macs3-project/ genomics-analysispipelines Bwa-mem version version 0.7.17 – http://arxiv.org/abs/1303.3997v2 Picard ‘‘Mark Duplicate’’ version 2.26.4 – https://broadinstitute.github.io/picard/ MACS3 version 3.0.0 Zhang et al. 50 – CHIPseeker version 1.30.0 Yu et al. 51 – Clusterprofiler version 4.2.0 Wu et al. 52 – Deeptools version 3.5.4 Ramirez et al. 53 – (Continued on next page) 20 Cell Reports 44, 116235, September 23, 2025 .. 6- to 8-week-old male outbred homozygous nude (Foxn1 nu /Foxn1 nu ) mice (strain 007850, Jackson Laboratory, Farmington, CT, USA) were used in xenograft studies.

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    Article Title: Disrupting the interaction between androgen receptor and its coregulator WDR77 delays the growth of treatment-resistant prostate cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 2000 Thermo-Fisher Scientific Cat# 11668019 Fugene HD Promega Cat# E2311 Glutathione Agarose Resin ThermoScientific Cat# 16101 Cytiva CM5 sensor Chip Cytiva Cat# BR100530 Octet® NTA Biosensors Sartorius Cat# 18-5101 Growth factor reduced phenol-free Matrigel Becton-Dickinson Cat# 356231 Matrigel matrix basement membrane Becton-Dickinson Cat# 356234 Critical commercial assays 1-Step Human High-Yield Mini IVT Kit Thermo-Fisher Scientific Cat# 88891 LookOut Mycoplasma PCR Detection Kit Sigma-Aldrich Cat# MP0035 CellTiter Aqueous One Solution Cell Proliferation Assays Promega Cat# G3580 Anti-DYDDDDK magnetic beads Pierce Cat# A36797 Anti-HA agarose beads Pierce Cat# 26181 NEBNext UltraII DNA library prep kit Illumina Cat# E7103S NEBNext UltraII library dual index primers Illumina Cat# E7600S Deposited data Raw and analyzed CUT&RUN seq data This paper GEO(GSE252993) RNAseq raw and analyzed files This paper GEO(GSE287925) Experimental models: Cell lines LNCaP ATCC Cat# CRL-1740 PC3 ATCC Cat# CRL-1435 DU145 ATCC Cat# HTB-81 C4-2 Uro-Cor – R1-D567 Scott Dehm laboratory, University of Minnesota – NIH3T3 Cell services core facility at Cleveland Clinic Lerner Research Institute – RWPE1 ATCC Cat# CRL-3607 RWPE2 ATCC Cat# CRL-3608 HEK293 Cell services core facility at Cleveland Clinic Lerner Research Institute – Experimental models: Organisms/strains homozygous nude (Foxn1nu/Foxn1nu) mice Jackson laboratory Strain 007850 Fox Chase SCID mouse Charles Rivers Laboratory Strain 236 Oligonucleotides Overview of primers used, included in Table S2 This paper N/A siRNAs targeting WDR77 (siGenome SmartPool) Horizon Discovery Cat#M-006895-01-0005 siRNAs targeting AR (siGenome SmartPool) Horizon Discovery Cat#M-003400-02-0005 Non-targeting control siRNAs (siGenome SmartPool) Horizon Discovery Cat#D-001810-10-20 Recombinant DNA p3xFLAG-CMV10 plasmid Sigma-Aldrich Cat# E7658 pT7CFE1-NHA-Chis ThermoFisher Scientific Cat# 88861 pT7CFE1-NFtag ThermoFisher Scientific Cat# 88865 pT7CFE1-NHis GST-CHA ThermoFisher Scientific Cat# 88871 (Continued on next page) Cell Reports 44, 116235, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER SMARTvector 2 inducible human WDR77targeting mCMV-turboGFP shRNA (sequences listed in Table S2) Horizon Discovery V3IHSMCG_4710023, V3IHSMCG_4967357, V3IHSMCG_8182514 SMARTvector inducible non-targeting control shRNA Horizon Discovery Cat# VSC6570 pT7CFE1-NHA-CHis-p53 This paper – pT7CFE1-Nflag-WDR77 This paper – pT7CFE1-Nflag-WDR77 1-107aa This paper – pT7CFE1-Nflag-WDR77 108-207aa This paper – pT7CFE1-Nflag-WDR77 208-342 This paper – pT7CFE1-Nis-GST-CHA-AR This paper – pT7CFE1-Nis-GST-CHA-AR-NTD This paper – pT7CFE1-Nis-GST-CHA-AR-DBD This paper – pT7CFE1-Nis-GST-CHA-AR-LBD This paper – pT7CFE1-Nis-GST-CHA-PRMT5 This paper – p3xFLAG-CMV10-WDR77 This paper – p3xFLAG-CMV10-WDR77 1-107aa This paper – p3xFLAG-CMV10-WDR77 108-207aa This paper – p3xFLAG-CMV10-WDR77 207-342aa This paper – p3xFLAG-CMV10-WDR77 SM This paper – p3xFLAG-CMV10-WDR77 DM This paper – p3xFLAG-CMV10-WDR77 peptide 3 This paper – pCW57.1 – Addgene David Root lab Cat# 41393 pCW57.1-WDR77 peptide 3 This paper – 3XHA-TurboID-NLS-pcDNA3 Kurt Runge lab, Cleveland Clinic – 3XHA-TurboID-NLS-pcDNA3-AR This paper – FUGW – Addgene Lois et al. 49 Cat# 14883 psPAX2 Abhishek Chakraborty lab, Cleveland Clinic – pMD2.G Abhishek Chakraborty lab, Cleveland Clinic – FUGW-WDR77-peptide 3 This paper – FUGW-WDR77-peptide 3 DM This paper – pCMV-AR Steven Balk laboratory, Beth Isreal Deaconess Medical Center – Software and algorithms Fiji (ImageJ) version 1.54 – https://imagej.net/software/fiji/ ChimeraX version 1.4 – https://www.rbvi.ucsf.edu/chimerax/ Image Studio (LI-COR) version 3.1 LI-COR https://www.licorbio.com/image-studio cBIO portal Cerami et al. 21 https://www.cbioportal.org/ Sigmaplot v16 – https://sigmaplot.software.informer.com/ 11.0/ Rstudio v2023.01 – https://www.r-project.org/ Prostate Cancer Transcriptome Atlas (PCTA) You et al. 27 http://www.thepcta.org/ Genomics Analysis pipeline – https://github.com/macs3-project/ genomics-analysispipelines Bwa-mem version version 0.7.17 – http://arxiv.org/abs/1303.3997v2 Picard ‘‘Mark Duplicate’’ version 2.26.4 – https://broadinstitute.github.io/picard/ MACS3 version 3.0.0 Zhang et al. 50 – CHIPseeker version 1.30.0 Yu et al. 51 – Clusterprofiler version 4.2.0 Wu et al. 52 – Deeptools version 3.5.4 Ramirez et al. 53 – (Continued on next page) 20 Cell Reports 44, 116235, September 23, 2025 .. 6- to 8-week-old male outbred homozygous nude (Foxn1 nu /Foxn1 nu ) mice (strain 007850, Jackson Laboratory, Farmington, CT, USA) were used in xenograft studies.

    Software:

    Article Title: Disrupting the interaction between androgen receptor and its coregulator WDR77 delays the growth of treatment-resistant prostate cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine 2000 Thermo-Fisher Scientific Cat# 11668019 Fugene HD Promega Cat# E2311 Glutathione Agarose Resin ThermoScientific Cat# 16101 Cytiva CM5 sensor Chip Cytiva Cat# BR100530 Octet® NTA Biosensors Sartorius Cat# 18-5101 Growth factor reduced phenol-free Matrigel Becton-Dickinson Cat# 356231 Matrigel matrix basement membrane Becton-Dickinson Cat# 356234 Critical commercial assays 1-Step Human High-Yield Mini IVT Kit Thermo-Fisher Scientific Cat# 88891 LookOut Mycoplasma PCR Detection Kit Sigma-Aldrich Cat# MP0035 CellTiter Aqueous One Solution Cell Proliferation Assays Promega Cat# G3580 Anti-DYDDDDK magnetic beads Pierce Cat# A36797 Anti-HA agarose beads Pierce Cat# 26181 NEBNext UltraII DNA library prep kit Illumina Cat# E7103S NEBNext UltraII library dual index primers Illumina Cat# E7600S Deposited data Raw and analyzed CUT&RUN seq data This paper GEO(GSE252993) RNAseq raw and analyzed files This paper GEO(GSE287925) Experimental models: Cell lines LNCaP ATCC Cat# CRL-1740 PC3 ATCC Cat# CRL-1435 DU145 ATCC Cat# HTB-81 C4-2 Uro-Cor – R1-D567 Scott Dehm laboratory, University of Minnesota – NIH3T3 Cell services core facility at Cleveland Clinic Lerner Research Institute – RWPE1 ATCC Cat# CRL-3607 RWPE2 ATCC Cat# CRL-3608 HEK293 Cell services core facility at Cleveland Clinic Lerner Research Institute – Experimental models: Organisms/strains homozygous nude (Foxn1nu/Foxn1nu) mice Jackson laboratory Strain 007850 Fox Chase SCID mouse Charles Rivers Laboratory Strain 236 Oligonucleotides Overview of primers used, included in Table S2 This paper N/A siRNAs targeting WDR77 (siGenome SmartPool) Horizon Discovery Cat#M-006895-01-0005 siRNAs targeting AR (siGenome SmartPool) Horizon Discovery Cat#M-003400-02-0005 Non-targeting control siRNAs (siGenome SmartPool) Horizon Discovery Cat#D-001810-10-20 Recombinant DNA p3xFLAG-CMV10 plasmid Sigma-Aldrich Cat# E7658 pT7CFE1-NHA-Chis ThermoFisher Scientific Cat# 88861 pT7CFE1-NFtag ThermoFisher Scientific Cat# 88865 pT7CFE1-NHis GST-CHA ThermoFisher Scientific Cat# 88871 (Continued on next page) Cell Reports 44, 116235, September 23, 2025 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER SMARTvector 2 inducible human WDR77targeting mCMV-turboGFP shRNA (sequences listed in Table S2) Horizon Discovery V3IHSMCG_4710023, V3IHSMCG_4967357, V3IHSMCG_8182514 SMARTvector inducible non-targeting control shRNA Horizon Discovery Cat# VSC6570 pT7CFE1-NHA-CHis-p53 This paper – pT7CFE1-Nflag-WDR77 This paper – pT7CFE1-Nflag-WDR77 1-107aa This paper – pT7CFE1-Nflag-WDR77 108-207aa This paper – pT7CFE1-Nflag-WDR77 208-342 This paper – pT7CFE1-Nis-GST-CHA-AR This paper – pT7CFE1-Nis-GST-CHA-AR-NTD This paper – pT7CFE1-Nis-GST-CHA-AR-DBD This paper – pT7CFE1-Nis-GST-CHA-AR-LBD This paper – pT7CFE1-Nis-GST-CHA-PRMT5 This paper – p3xFLAG-CMV10-WDR77 This paper – p3xFLAG-CMV10-WDR77 1-107aa This paper – p3xFLAG-CMV10-WDR77 108-207aa This paper – p3xFLAG-CMV10-WDR77 207-342aa This paper – p3xFLAG-CMV10-WDR77 SM This paper – p3xFLAG-CMV10-WDR77 DM This paper – p3xFLAG-CMV10-WDR77 peptide 3 This paper – pCW57.1 – Addgene David Root lab Cat# 41393 pCW57.1-WDR77 peptide 3 This paper – 3XHA-TurboID-NLS-pcDNA3 Kurt Runge lab, Cleveland Clinic – 3XHA-TurboID-NLS-pcDNA3-AR This paper – FUGW – Addgene Lois et al. 49 Cat# 14883 psPAX2 Abhishek Chakraborty lab, Cleveland Clinic – pMD2.G Abhishek Chakraborty lab, Cleveland Clinic – FUGW-WDR77-peptide 3 This paper – FUGW-WDR77-peptide 3 DM This paper – pCMV-AR Steven Balk laboratory, Beth Isreal Deaconess Medical Center – Software and algorithms Fiji (ImageJ) version 1.54 – https://imagej.net/software/fiji/ ChimeraX version 1.4 – https://www.rbvi.ucsf.edu/chimerax/ Image Studio (LI-COR) version 3.1 LI-COR https://www.licorbio.com/image-studio cBIO portal Cerami et al. 21 https://www.cbioportal.org/ Sigmaplot v16 – https://sigmaplot.software.informer.com/ 11.0/ Rstudio v2023.01 – https://www.r-project.org/ Prostate Cancer Transcriptome Atlas (PCTA) You et al. 27 http://www.thepcta.org/ Genomics Analysis pipeline – https://github.com/macs3-project/ genomics-analysispipelines Bwa-mem version version 0.7.17 – http://arxiv.org/abs/1303.3997v2 Picard ‘‘Mark Duplicate’’ version 2.26.4 – https://broadinstitute.github.io/picard/ MACS3 version 3.0.0 Zhang et al. 50 – CHIPseeker version 1.30.0 Yu et al. 51 – Clusterprofiler version 4.2.0 Wu et al. 52 – Deeptools version 3.5.4 Ramirez et al. 53 – (Continued on next page) 20 Cell Reports 44, 116235, September 23, 2025 .. 6- to 8-week-old male outbred homozygous nude (Foxn1 nu /Foxn1 nu ) mice (strain 007850, Jackson Laboratory, Farmington, CT, USA) were used in xenograft studies.



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    paper pcw57 1 addgene david root lab  (Addgene inc)
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    Addgene inc paper pcw57 1 addgene david root lab
    Paper Pcw57 1 Addgene David Root Lab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paper+pcw57+1+addgene+david+root+lab/pCW57%2E1+(Plasmid+%2341393)/pm40934084-277-107-111
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    paper pcw57 1 addgene david root lab - by Bioz Stars, 2026-11
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